R&D priorities in biotechnology are essential to take care of post-Kyoto challenges:
A. Global Warming: The third session of the Conference of the Parties to the United Nations Framework Convention on Climate change, held in Kyoto, Japan, on December 1997, agreed on a protocol which includes each party’s quantitative commitment to reduce its emissions of greenhouse gases, such as carbon dioxide (CO2) by 2010. The protocol specifies that the European Union will commit itself to reducing its greenhouse gas emissions by 8 per cent by 2010 from the level of 1990 (base year), the United States by 7 per cent, and Japan and Canada by 6 per cent. As an essential element in achieving this goal, industry must reduce energy consumption in order to maintain development while helping to meet these targets.
This would include a shift from present petrochemical industry processes, which consume large quantities of energy under conditions of high temperature and pressure, to more energy-efficient biological processes, which use renewable resources such as biomass to produce useful substances under normal temperatures and pressures. For example, future processes will focus more on producing efficiently alternative fuels such as ethanol, which contribute less to global warming and are also likely to produce environmentally benign products, such as biodegradable plastics, which breaks down in natural settings after use.
As a result, biotechnology should become an increasingly valuable tool for developing environmentally friendly products and processes and for preventing the Earth from warming.
B. R&D priorities in biotechnology for promotion of clean industrial products and processes: If biotechnology is to become an increasingly important source of clean industrial products and processes, R&D efforts will need to focus on a number of priority areas. Among those that deserve prompt and focused research in the near future are:
a. Innovative products derived from biological sources that contribute to sustainability;
b. Wider exploration of biological systems (enzymes, micro-organisms, cells, whole organisms);
c. Greater emphasis on the use of bioconsortia, including establishing them and developing production and degradation processes based on them;
d. Novel methodologies for developing biological processes (bio-molecular design, genomics);
e. Innovative biocatalyst technology for use in areas where conventional biocatalysts have not yet been exploited (e.g. the petrochemical industries);
f. Biological recycling processes that convert unused resources to useful substances;
g. Emphasis on engineering, especially large-scale engineering, process intensification, measurement, monitoring and control systems;
h. Greater emphasis on biodiversity and widening the search for novel genes (bioprospecting), a process that will require, in parallel, the construction of infrastructures such as culture collections, comprehensive biological databases, and the development of bioinformatics;
i. Focus on development and application of recombinant technology.
Biotechnology and industrial sustainability in producing clean industrial products:
A. Introduction: Various points have been given below regarding role of biotechnology and industrial sustainability in producing clean industrial products:
a. Industrial sustainability demands a global vision and co-ordinated policy approaches. b. In an industrial context, sustainability is equated with clean industrial products and processes. c. Biotechnology is competitive with and in many cases complements chemical methods for achieving clean technologies. d. It is essential to determine what is clean or cleaner, using Life Cycle Assessment and related methods. e. Biotechnology is a versatile enabling technology that provides powerful routes to clean industrial products and processes and is expected to play a growing role.
B. Biotechnology and CO2 emissions: Fossil carbon represents the single most important raw material for energy generation and for chemicals manufacture, but its oxidation product, CO2, is an important greenhouse gas. Any means of reducing fossil carbon consumption, either by improving energy efficiency or by substituting alternative resources will directly result in lowered CO2 production and thus reduce global warming.
C. Industrial processes: Use of biotechnology has already resulted in energy reduction in industrial processes. In only a few instances can the reductions be quantified, and these are presented in this report. Others are only available as anecdotal evidence. As yet, there are insufficient data to allow scaling up these figures to cover whole industrial sectors.
Examples: a. Ammonium acrylate, a key intermediate in the manufacture of acrylic polymers, is made by hydrolyzing acrylonitrile to acrylic acid and reacting this with ammonia. The reaction is energy-intensive and gives rise to by-products which are difficult to remove. A process, based on a bacterial enzyme which directly synthesises ammonium acrylate of the same quality under less energy-demanding conditions, has been operating for several years at full scale.
b. In paper making, treating cellulose fibres in the pulp using cellulase and hemicellulase enzymes allows water to drain more quickly from the wet pulp, thereby reducing processing time and energy used for drying. Trials have shown that machine speeds can be increased by up to 7 per cent and energy input reduced by as much as 7.5 per cent. Replacing thermomechanical pulping by biopulping has resulted in up to 30 per cent reduction in electrical energy consumption.
D. Materials: Biomass, as it grows, consumes CO2. Substances made from such renewable raw materials are therefore a zero net contributor to atmospheric greenhouse gases, unless fossil fuel is used in their manufacture. A wide range of chemicals and structural materials can be based on biological raw materials including biodegradable plastics, biopolymers and biopesticides, novel fibres and timbers. Plant-derived amides, esters and acetates are currently being used as plasticisers, blocking/slip agents and mould-release agents for synthetic polymers. Uses of biohydrocarbons linked to amines, alcohols, phosphates and sulphur ligands include fabric softeners, corrosion inhibitors, ink carriers, solvents, hair conditioners, and perfumes.
E. Chemicals from biological feedstocks: It is no longer necessary to start with a barrel of oil to produce chemicals. Corn, beets, rice – even potatoes – make excellent feedstocks. The fact that micro-organisms transform sugars into alcohol has been known for a very long time. But only since the advent of genetic engineering is it feasible to think about harnessing the sophistication of biological systems to create molecules that are difficult to synthesise by traditional chemical methods.
For example, the polymer polytrimethylene terephthalate (3GT) has enhanced properties compared to traditional polyester (2GT). Yet commercialization has been slow to come because of the high cost of making trimethylene glycol (3G), one of 3GT’s monomers. The secret to producing 3G can be found in the cellular machinery of certain unrelated microorganisms. Some naturally occurring yeasts convert sugar to glycerol, while a few bacteria can change glycerol to 3G. The problem is that no single natural organism has been able to do both. Through recombinant DNA technology, an alliance of scientists from DuPont and Genencor International has created a single micro-organism with all the enzymes required to turn sugar into 3G. This breakthrough is opening the door to low-cost, environmentally sound, large-scale production of 3G. The eventual cost of 3G by this process is expected to approach that of ethylene glycol (2G). The 3G fermentation process requires no heavy metals, petroleum or toxic chemicals. In fact, the primary material comes from agriculture – glucose from cornstarch. Rather than releasing carbon dioxide to the atmosphere, the process actually captures it because corn absorbs CO2 as it grows. All liquid effluent is easily and harmlessly biodegradable. Moreover, 3GT can readily undergo methanolysis, a process that reduces polyesters to their original monomers. Post-consumer polyesters can thus be repolymerised and recycled indefinitely.
F. Clean fuels: While biomass can be consumed (incinerated) directly to produce energy, it can also be converted into a wide range of chemicals and liquid fuels. Although, in energy terms, annual land production of biomass is some five times global energy consumption, biomass presently provides only 1 per cent of commercial energy. Biomass energy cannot compete at present-day prices with fossil fuels and has so far penetrated the market only where governments have effectively subsidised its use. Bioethanol is a CO2-neutral alternative liquid transportation fuel. As new technologies – including continuous fermentation, production from lignocellulosic (wood and agricultural crop) waste – and more efficient separation techniques are developed, the cost of bioethanol will compete with that of gasoline. Over a 20-year period, US ethanol production, based solely on lignocellulosic waste, could rise to 470 million tonnes a year, equal to present gasoline consumption in energy terms.
Biotechnology for development of sustainable clean technology - Strategies:
Many developed countries started using biotechnology as a means of achieving clean or cleaner industrial products and processes. It compares biotechnological processes with competing means of securing similar goals.
Meaning of Clean technology - All stages of the life cycle of a product or process may adversely affect the environment by using up limited resources of materials and energy or by creating waste. Any substitution or change that reduces consumption of materials and energy and production of waste – including, for example, recycling of materials and energy – may be regarded as more environmentally friendly or ‘‘clean’’. Clean technology may also be equated with reduced risk.
Life Cycle Assessment is one way of comparing the relative cleanliness of a product or process.
Cleaner processes and products mean processes and products that consume less energy and material resources, generate less pollution or waste, or use renewable resources rather than petroleum or coal-based feedstock as feed. There are many reasons why an operator would switch to a cleaner process or product. Some of the more important factors most often mentioned are:
(a) Availability of raw materials;
(b) Cost factors;
(c) Market demands;
(d) Safety and health considerations;
(e) Environmental considerations;
(f) Product liability;
(g) Public image.
Thus, it is the duty of developed countries to appreciate the potential role of biotechnology in clean industrial processes and sets the stage for viewing clean processes in the context of industrial sustainability. The extents to which biotechnological thinking and practices are being introduced into industrial sectors, which have serious environmental impacts, are to be enhanced. The economic competitiveness of biotechnology for clean products and processes in these sectors are the major concerns, which Government / authorities required to be take a note and policy implementation should be in tune with sustainable development.
Scientific and technological innovations across the range of biotechnologies and the opportunities for their adoption, as well as R&D priorities are to be spelt out.
The following few points are to be kept in mind while framing strategies for promotion of biotechnology for development of clean technology, in the context of industrial sustainability:
(a) Global environmental concerns will drive increased emphasis on clean industrial products and processes.
(b) Biotechnology is a powerful enabling technology for achieving clean industrial products and processes that can provide a basis for industrial sustainability.
(c) Measuring the cleanliness of an industrial product or process is essential but complex; Life Cycle Assessment (LCA) is the best current tool for making this determination.
(d) The main drivers for industrial biotechnological processes are economic (market forces), government policy, and science and technology.
(e) Achieving greater penetration of biotechnology for clean environmental purposes will require joint R&D efforts by government and industry.
(f) For biotechnology to reach its full potential as a basis for clean industrial products and processes, beyond its current applications, additional R&D efforts will be needed.
(g) Because biotechnology, including recombinant DNA technology and its applications, has become increasingly important as a tool for creating value-added products and for developing biocatalysts, there is a strong need for harmonised and responsive regulations and guidelines.
(h) Market forces can provide very powerful incentives for achieving environmental cleanliness objectives.
(i) Government policies to enhance cleanliness of industrial products and processes can be the single most decisive factor in the development and industrial use of clean biotechnological processes.
(j) Communication and education will be necessary to gain penetration of biotechnology for clean products and processes into various industrial sectors.
Environmental protection using Biotechnology – An overview:
A. The surroundings around us are termed as ‘environment’. Our environment includes the abiotic component (the non living) and biotic component (the living). The abiotic environment includes air, water and soil; and the biotic environment consists of all living organisms such as plants, animals and microorganisms. Environmental pollution broadly refers to the presence of undesirable substances in the environment which are harmful to man and other organisms. There has been a significant increase in the levels of harmful environmental pollution mostly due to direct or indirect human activities in recent past. The major sources of environmental pollution are industries, agricultural and other anthropogenic and biogenic sources etc. The pollutants are chemical, biological and physical in nature.
B. Controlling the environmental pollution and the conservation of environment and biodiversity and controlling environmental pollution are the major focus areas of all the countries around the world. In this context, the importance and impact of biotechnological approaches and the implications of biotechnology has to be thoroughly evaluated. There have been serious concerns regarding the use of biotechnological products and the impact assessment of these products due to their interaction with the environmental factors. A lobby of the environmentalists has expressed alarm on the release of genetically engineered organisms in the atmosphere and have stressed on thorough investigation and proper risk assessment of theses organisms before releasing them in to the environment. The effect of the effluents from biotechnological companies is also a cause of concern for everyone. The need of the hour is to have a proper debate on the safety of the use of the biotechnological products. The efforts are not only on to use biotechnology to protect the environment from pollution but also to use it to conserve the natural resources. As we all know that microorganisms are known natural scavengers so the microbial preparations (both natural as well as genetically engineered) can be used to clean up the environmental hazards.
C. Biotechnology is being used to provide alternative cleaner technologies which help to further reduce the hazardous environmental implications of the traditional technologies. Some of the well known examples and mechanisms are:
(i) Some fermentation technologies have some serious environmental implications. Various biotechnological processes have been devised in which all nutrients introduced for fermentation are retained in the final product, which ensures high conversion efficiency and low environmental impact.
(ii) In paper industry, the pulp bleaching technologies are being replaced by more environmentally friendly technologies involving biotechnology. The pulp processing helps to remove the lignin without damaging valuable cellulosic fibres but the available techniques suffer from the disadvantages of high costs, high energy use and corrosion. A lignin degrading and modifying enzyme (LDM) was isolated from Phanerochaete chrysosporum and was used, which on one hand, helped to reduce the energy costs and corrosion and on the other hand increased the life of the system. This approach helped in reducing the environmental hazards associated with bleach plant effluents.
(iii) In Plastic industry, the conventional technologies use oil based raw materials to extract ethylene and propylene which are converted to alkene oxides and then polymerized to form plastics such as polypropylene and polyethylene. There is always the risk of these raw materials escaping into the atmosphere thereby causing pollution. Using biotechnology, more safer raw materials like sugars (glucose) are being used which are enzymatically or through the direct use of microbes converted into alkene oxides.e.g. Methylococcus capsulatus has been used for converting alkene into alkene oxides.
(iv) Bioremediation is defined as ‘the process of using microorganisms to remove the environmental pollutants where microbes serve as scavengers. The removal of organic wastes by microbes leads to environmental cleanup. The other names/terms used for bioremediation are bio-treatment, bio-reclamation, and bio-restoration. The term “Xenobiotics” (xenos means foreign) refers to the unnatural, foreign and synthetic chemicals such as pesticides, herbicides, refrigerants, solvents and other organic compounds. The microbial degradation of xenobiotics also helps in reducing the environmental pollution. Depending on the method followed to clean up the environment, the bioremediation is carried out in two ways:
(a) In situ bioremediation – involves a direct approach for the microbial degradation of xenobiotics at the site of pollution which could be soil, water etc. The in situ bioremediation is generally used for clean up of oil spillages, beaches etc.;
(b) Ex-situ bioremediation - In this the waste and the toxic material is collected from the polluted sites and the selected range of microorganisms carry out the bioremediation at designed place. This process is an improved method over the in situ bioremediation method.
(v) Pseudomonas which is a soil microorganism effectively degrades xenobiotics. Different strains of Pseudomonas that are capable of detoxifying more than 100 organic compounds (e.g. phenols, biphenyls, organophosphates, naphthalene etc.) have been identified. Some other microbial strains are also known to have the capacity to degrade xenobiotics such as Mycobacterium, Alcaligenes, Norcardia etc.
D. In recent years, efforts have been made to create genetically engineered microorganisms to enhance bioremediation. This is done to overcome some of the limitations and problems in bioremediation. These problems are: a) Sometimes the growth of microorganisms gets inhibited or reduced by the xenobiotics. b) No single naturally occurring microorganisms has the capability of degrading all the xenobiotics present in the environmental pollution. c) The microbial degradation is a very slow process. d) Sometimes certain xenobiotics get adsorbed on to the particulate matter of soil and thus become unavailable for microbial degradation.
E. As the majority of genes responsible for the synthesis of enzymes with biodegradation capability that are located on the plasmids, the genetic manipulations of plasmids can lead to the creation of new strains of bacteria with different degradative pathways. Well known example of genetic manipulations of plasmids is development of ‘Superbug’, which is used for degrading a number of hydrocarbons of petroleum simultaneously such as camphor, octane, xylene, naphthalene etc.
F. We all know that, carbon dioxide (CO2) is the main cause of green house effect and rise in the atmospheric temperature. There is a steady increase in the CO2 content due to continuous addition of CO2 from various sources particularly from industrial processes. It is very clear that the reduction in atmospheric CO2 concentration assumes significance. Biotechnological methods have been used to reduce the atmospheric CO2 content at two levels:
(a) Photosynthesis- Plants utilize CO2 during the photosynthesis which reduces the CO2 content in the atmosphere;
(b) Biological Calcification- Certain deep sea organisms like corals, green and red algae store CO2 through a process of biological calcification. As the CaCO3 gets precipitated, more and more atmospheric CO2 can be utilized for its formation.
G. The sewage is treated to get rid of these undesirable substances by subjecting the organic matter to biodegradation by microorganisms. The biodegradation involves the degradation of organic matter to smaller molecules, such as CO2, NH3, PO4 etc., and requires constant supply of oxygen. The process of supplying oxygen is expensive, tedious, and requires a lot of expertise and manpower. These problems are overcome by growing micro-algae in the ponds and tanks where sewage treatment is carried out. The algae release the O2 while carrying out the photosynthesis which ensures a continuous supply of oxygen for biodegradation. The algae are also capable of adsorbing certain heavy toxic metals due to the negative charges on the algal cell surface which can take up the positively charged metals. The algal treatment of sewage also supports fish growth as algae are a good source of food for fishes.
H. The environmental impact assessment system requires proponents to foresee possible environmental impacts when a development project is being planned, and to conduct an environmental assessment. However, debate continues on exactly what kinds of environmental protection measures are needed and how they should be integrated into a given project to achieve desirable environmental results. Actions to deal with global warming and to prevent ozone layer depletion are gaining momentum, but currently available technologies may not be enough to meet the required targets. Technological advances are needed in order to make progress in solving these issues, as well as with the problem of dioxins. New developments are also needed in technologies for pollution removal and environmental restoration, in cases where environmental pollution has already been generated or is already accumulating in the environment.
Environmental biotechnology – serving the future
Like white biotechnology, environmental biotechnology, often referred to as “grey biotechnology”, also focuses on sustainability. For instance, environmental biotechnology deals with the treatment of sewage water, the purification of exhaust gas or the decontamination of soils or ground water using specific microorganisms.
The use of organisms for the removal of contamination or pollutants is generally referred to as bioremediation. Originally, bioremediation was mainly used in cleanup operations, including the decomposition of spilt oil or slagheaps containing radioactive waste. In addition, bioremediation is also the method of choice when solvents, plastics or heavy metals and toxic substances like DDT, dioxins or TNT need to be removed.
Bioadsorption processes using newly developed bioadsorbers made from renewable materials are currently being developed. These adsorbers function as ion exchangers and are used in the elimination and disposal of toxic heavy metals. The industrial use of mineral resources leads to the drastic accumulation of these pollutants in the biosphere. The new bioadsorbers are used for the elimination of heavy metals and radionuklids from industrial wastewater, ore mine wastewater, seepage water from dumpsites or wastewater from nuclear power stations.
Biotechnology to address many global environmental concerns:
A. Industrial biotechnology has come of age. Improved industrial sustainability through biotechnology addresses many global environmental concerns. Biotechnology has clear environmental advantages and is economically competitive in a growing number of industrial sectors. It enables reductions of material and energy consumption, as well as pollution and waste generation, for the same level of industrial production. Continued technical innovation, including that based upon recombinant DNA technology, is vital for the wider utilisation of biotechnology by industry.
B. With biotechnology, the emphasis is no longer on the removal of pollutants from an already damaged environment, but on the need to reshape industrial process technologies to prevent pollution at the source. Achieving ‘‘clean technology’’ or ‘‘industrial sustainability’’ – the two terms are largely congruent – will not be possible without a steady stream of creative innovations based on advanced science and technologies, among which biotechnology is likely to play an increasing role.
C. Although definitions of sustainable development have frequently proved elusive, it is clear that any move towards industrial sustainability will affect all stages of a product’s or process’s life cycle. It will require new design principles based on a global and holistic approach to reducing environmental impacts: global because these impacts transcend national borders, holistic because short-term, piecemeal solutions to address a succession of issues in isolation will be less and less effective. One important means of integrating environmental issues into industrial design and operations is the adoption of Life Cycle Assessment (LCA).
D. There are three main drivers of clean technology: (a) Economic competitiveness, with companies considering the advantages of clean products and processes in terms of market niches or cost advantages; (b) Government policies, which enforce or encourage changes in manufacturing practices; and (c) Public pressure, which takes on strategic importance as companies seek to establish environmental legitimacy.
E. It is possible to foresee a growing role for industrial process biotechnology, both because it may afford clear economic and environmental benefits, and because the power of the tool itself continues to grow. The expectations of greater cleanliness come from the observation that living systems manage their chemistry rather more efficiently than man-made chemical plants, and that their wastes tend to be recyclable and biodegradable. This, along with our increasing ability to manipulate biological materials and processes, strongly points to a significant impact on the future of manufacturing industries.
F. Here there is a brief picture of how modern process biotechnology is penetrating industrial operations:
(i) Biotechnology embraces a wide range of techniques, and none of these will apply across all industrial sectors. Nonetheless, the technology is so versatile that many industries that have not used biological sciences in the past are now exploring the possibility of doing so. Already, the economic competitiveness of a variety of biotechnological applications to achieve cleanliness has been established. This is essential, as environmental benefits alone have seldom driven the adoption of biotechnology-based processes. Such processes have been successfully integrated into some large-scale operations. However, a number of problems remain for industrial applications, particularly the entrenched infrastructure of companies that have traditionally relied on physical and chemical technology alone and whose engineers have no training in life sciences or technologies.
(ii) Chemicals manufacturing is a major generator of materials, a major consumer of energy and non-renewable resources, and a major contributor to waste and pollution. In these sub-sectors, market penetration of biotechnology varies. It is in the fine chemical industries that the impact of clean biotechnology is most visible.
(iii)While fossil carbon (oil, coal) is the single most important raw material for energy generation and for chemicals, the concomitant CO2 emissions are a source of increasing concern because CO2 is a major greenhouse gas. Biotechnology can contribute to reducing fossil carbon consumption and hence global warming in various ways: improving industrial processes and energy efficiency, and producing biomass-based materials and clean fuels.
(iv) In pulp and paper, market penetration of biotechnology used for clean production is particularly high in many of the developed nations, and biotechnology is becoming more important in the manufacture of textiles and leather throughout the western world.
(v) In the food and feed sector, the impact of biotechnology on clean industrial processes seems to be greatest in the United States.
(vi) Biotechnology for mining and metals recovery covers two major technologies: bioleaching/minerals bio-oxidation, where superior cleanliness and economic profitability have been claimed in specific cases, and metals bioremediation and recovery.
(vii) In the energy sector, biotechnology has had a major effect both on economics and on environmental impacts. It has improved the overall efficiency of processes, particularly in the area of pollution control. Processes currently under development, such as bio-diesel, bio-ethanol and bio-desulphurisation, seek to replace energy-intensive and polluting systems with systems that are more environmentally friendly. The effect of rDNA methods on these technologies will be great, but large-scale application of rDNA has only recently begun and has not yet had dramatic effects.
G. Although the potential of biotechnology to reduce raw materials and energy consumption as well as wastes is attractive, there is a need for further encouragement, notably by government, particularly when the economic advantages are not overwhelming in the early stages of adoption.
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